iPPHOT: A webserver for predicting hotspots at protein-protein interfaces


A webserver for identifying protein-protein interfaces hotspot residues. iPPHOT was developed by using support vector machine based on three different kinds of features including relative consevation, solvent accessible surface area, and B factors of residues. It needs three input files: pdbfile which contains the coordinates of a protein-protein complexes; consurf.grades and msa_aa_variety_percentage.csv that was generated by the ConSurf server. More details can be found in the tutorial.

Citation: Qiao Y, Xiong Y, Gao H, Zhu X,Chen P. Protein-protein interface hot spots prediction based on a hybrid feature selection strategy. BMC Bioinformatics 2018; 19(1):14. Doi: 10.1186/s12859-018-2009-5

Input Form

PDB File Upload:

Chain id:

consurf.grades generated by ConSurf server:

msa_aa_variety_percentage.csv generated by ConSurf server: